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Product Name：COVID-19 Nucleic Acid RT-PCR Test Kit

Specification：20 Tests/Kit (SC-COVID19-20)、100 Tests/Kit (SC-COVID19-100)

Sample：throat swabs, nasal swabs, nasopharyngeal, and oropharyngeal swabs specimens

Principles of the Procedure

The COVID-19 Nucleic Acid RT-PCR Test Kit is a real-time reverse transcription polymerase chain reaction (rRT -PCR) test. The COVID-19 primer and probe set(s) is designed to detect RNA from the COVID-19 in throat swabs, nasal swabs, nasopharyngeal, and oropharyngeal swabs specimens from patients with signs and symptoms of infection who are suspected of COVID-19.

This kit uses specific primers and TaqMan probes (5’ end-labeled FAM, VIC or ROX fluorescent probes) to simultaneously amplify the COVID-19 ORF1ab gene, N gene fragments, and actin gene in one tube. In the case where the COVID-19 RNA template is contained in the reaction system, a fluorescent signal is released during the PCR amplification process. The instrument is used to monitor the signal intensity of the corresponding channel in real time during the PCR process to achieve qualitative detection results.

This kit uses dUTP and uracil-DNA N-glycosylase (UNG) to minimize PCR cross-contamination from previously amplified PCR products. To avoid possible false positive results, the reaction mixture undergoes a 2 min incubation at 37 °C prior to PCR amplification, allowing UNG to fully degrade potential PCR product contamination.

The kit uses the human β-actin gene as an internal reference gene. This internal control is detected using specific primers and probes (5’end-labeled ROX fluorescent probes) in order to exclude false negative results via confirming the success of the reaction in real time.